감껍질, 여주, 작두콩 추출물이 Streptococcus mutans의 우식유발 관련특성에 미치는 영향
Effects of Diospyros kaki peel, Momordica charantia, and Canavalia gladiata extracts on the cariogenic traits of Streptococcus mutans
김동운, 이동익, 임상욱, 최연희, 송근배,
소속 상세정보
김동운 ( Kim Dong-Woon ) - 경북대학교 치과대학 예방치과학교실
이동익 ( Lee Dong-Ik ) - 경북대학교 치과대학 예방치과학교실
임상욱 ( Im Sang-Uk ) - 경북대학교 치과대학 예방치과학교실
최연희 ( Choi Youn-Hee ) - 경북대학교 치과대학 예방치과학교실
송근배 ( Song Keun-Bae ) - 경북대학교 치과대학 예방치과학교실
Abstract
Objectives: The purpose of this study is to determine methods of dental caries prevention by investigating the use of compounds of Diospyros kaki (D. kaki) peel, Momordica charantia (M. charantia), and Canavalia gladiata (C. gladiata) extracts to limit the cariogenic traits of Streptococcus mutans (S. mutans), such as their ability to proliferate and adhere to the tooth surface.
Methods: Broth microdilution and the agar spreading assay were used to determine the antimicrobial effect and minimum inhibitory concentration (MIC) of S. mutans extracts. In order to identify the adhesive ability of S. mutans at varying concentrations, culture plates were first stained with 1 ml of 0.01% crystal violet for 15 minutes at room temperature, and then eluted with 1 ml of EtOH:Acetone (8:2) solution for 15 minutes in a 37°C incubator. Eluted solutions were then evaluated by use of a spectrophotometer at 575 nm.
Results: Experiments were conducted in order to investigate the effectiveness of D. kaki peel, M. charantia, and C. gladiata extracts on limiting the proliferation of S. mutans. The MIC was measured as an indication of whether the antibacterial activity of D. kaki peel, M. charantia, and C. gladiata extracts had a significant bacteriostatic effect on S. mutans. M. charantia extract was effective for growth inhibition on S. mutans at a minimum concentration of 0.25%. From the adhesion ability assay, M. charantia extract had an anti-adhesive effect.
Conclusions: These results indicate that M. charantia extract demonstrates antibacterial activity and has an anti-adhesive effect on S. mutans. Due to these properties, M. charantia extract may be used to prevent dental caries.
키워드
Antibacterial activity; Anti-adhesive effect; Canavalia gladiata; Diospyros kaki peel; Momordica charantia; Streptococcus mutans
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